Research & Sources

GH, KH & pH: Research & Sources

This research record shows the studies, veterinary references, aquaculture guidance, technical documentation, and aquarium sources used behind The Tank Guide’s GH, KH, and pH content. It also shows where evidence is strong, where a source only partially supports a claim, and where historical citations could not be independently recovered.

Audit complete · documented limitations Last audited:

The purpose of this page is transparency. Listing a source here does not mean every source carries the same scientific weight — a peer-reviewed physiology study, a veterinary manual, a product page and a forum thread are very different kinds of evidence, and this record labels them that way.

Part of The Tank Guide University.

What this research record covers

The Tank Guide assembled one master research package on GH, KH and pH in freshwater aquariums (compiled August 2026). It is a 94-page reference compilation, not an article. Its core idea: each parameter answers a different question — pH is how acidic the water is right now, GH is how much calcium and magnesium is dissolved, and KH is how much acid the water can absorb before pH moves. The package covers:

  • What pH measuresThe logarithmic scale, how pH is measured, and why it swings over a day.
  • General hardness (GH)Calcium and magnesium, units, and their roles in fish and invertebrates.
  • Carbonate hardness (KH) / alkalinityTerminology, units, and how KH is used up and replenished.
  • How GH, KH and pH relateCommon water profiles and why one number doesn’t predict the others.
  • Buffering and pH stabilityLow-KH systems, and a look at “stable pH matters more than the right pH” and “don’t chase pH.”
  • Soft-water and hard-water fishSpecies requirements, osmoregulation, and survival vs. thriving vs. breeding.
  • Source waterTap, well, softened and blended water.
  • RO/DI water and remineralizationWhy pure RO water isn’t aquarium water on its own.
  • Adjusting GH, KH and pHMethods, dosing arithmetic and risks — including driftwood, botanicals, crushed coral and aquasoils.
  • Planted tanks and CO₂The limitations of the common pH/KH/CO₂ table.
  • Nitrification and Old Tank SyndromeHow the nitrogen cycle consumes alkalinity, and gradual pH decline in older tanks.
  • Testing, units and conversionsIncluding TDS and conductivity.
  • Shrimp, snails and plantsIncluding where common shrimp parameter targets are hobby convention.
  • Care-sheet ranges, acclimation and mythsWhere commonly repeated numbers and claims come from, and how well they hold up.

This is a summary of the package’s scope. It is not a new set of care recommendations — for practical guidance, read the articles below.

Tank Guide content developed from this research

These pieces were developed using this research package.

Production relationships were confirmed by the creator of The Tank Guide.

Evidence at a glance

Every bibliography source was checked on its own, and every link between a source and a statement in the package was reviewed separately.

43 bibliography sources reviewed

  • 8Verified
  • 30Verified with citation/metadata corrections
  • 1Verified with a scope limitation
  • 4Could not be independently verified

“Could not be independently verified” means the historical source was sought, but the original material could not be reliably recovered or inspected. It is not a judgment that the source was bad.

68 source-to-claim relationships reviewed

  • 31Direct support
  • 10Partial support
  • 1Citation mismatch / unsupported
  • 5Manufacturer documentation
  • 1Background support
  • 13Bibliography-only
  • 7Historical attribution still limited

A single source can support several statements, so there are more relationships than sources. Labels are explained just below.

How to read this research record

Direct support
The source directly supports the specific statement attributed to it.
Partial support
The source supports part of the statement, but the package wording is broader.
Background support
The source is relevant context but is not direct proof of the full claim.
Manufacturer documentation
The source verifies what a manufacturer says about its own product. It is not independent scientific validation.
Bibliography-only
The historical package listed the source but did not clearly connect it to a specific statement.
Historical attribution limited
The relationship was reviewed, but the surviving information is not sufficient for stronger attribution.
Citation mismatch
The cited source does not support the claim attributed to it.

Each source also carries a verification label: Verified; Verified · citation corrected (the source was confirmed, but details such as authors, journal, year or URL needed correcting); Verified · limited scope; or Could not be independently verified.

Source library

All 43 sources from the package bibliography, grouped the way the original package grouped them by type of evidence. Each entry also shows its audited source type and verification result. Open a group to see its sources; links go to the verified source and open in a new tab.

Peer-reviewed scientific literature

14 sources

Journal articles and scholarly reviews in fish physiology — mostly research on how calcium, low pH and dissolved organic carbon affect ion balance at the gills. This is the strongest class of evidence in the record, although much of it studies wild Amazonian or model species rather than home aquariums.

Show all 14 sources in Peer-reviewed scientific literature
  1. Dissolved organic carbon from the upper Rio Negro protects zebrafish (Danio rerio) against ionoregulatory disturbances caused by low pH exposure

    Author or organization: Duarte, Rafael M.; Smith, D. Scott; Val, Adalberto L.; Wood, Chris M.

    Published in: Scientific Reports, 6, 20377 · 2016

    DOI: 10.1038/srep20377

    • Peer-reviewed journal article
    • Verified

    Reviewed relationships: 3 direct support.

  2. Protection by Natural Blackwater against Disturbances in Ion Fluxes Caused by Low pH Exposure in Freshwater Stingrays Endemic to the Rio Negro

    Author or organization: Wood, Chris M.; Matsuo, Aline Y. O.; Wilson, Rod W.; Gonzalez, R. J.; Patrick, Marjorie L.; Playle, Richard C.; Val, Adalberto Luis

    Published in: Physiological and Biochemical Zoology, 76(1), 12–27 · 2003

    DOI: 10.1086/367946

    • Peer-reviewed journal article
    • Verified

    Reviewed relationships: 2 direct support.

  3. The physiology of fish in acidic waters rich in dissolved organic carbon, with specific reference to the Amazon basin: Ionoregulation, acid–base regulation, ammonia excretion, and metal toxicity

    Author or organization: Morris, Carolyn; Val, Adalberto L.; Brauner, Colin J.; Wood, Chris M.

    Published in: Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, 335(9–10), 843–863 · 2021

    DOI: 10.1002/jez.2468

    • Peer-reviewed journal article
    • Verified

    Reviewed relationships: 1 direct support.

  4. Diverse Strategies for Ion Regulation in Fish Collected from the Ion-Poor, Acidic Rio Negro

    Author or organization: Gonzalez, R. J.; Wilson, R. W.; Wood, C. M.; Patrick, M. L.; Val, A. L.

    Published in: Physiological and Biochemical Zoology, 75(1), 37–47 · 2002

    DOI: 10.1086/339216

    • Peer-reviewed journal article
    • Verified · citation corrected

    Reviewed relationships: 1 partial support.

    Original package citation corrected during verification: the package’s author list omitted R. W. Wilson and listed the remaining authors in a different order.

  5. Ionoregulation in Tropical Fishes from Ion-Poor, Acidic Blackwaters

    Author or organization: Gonzalez, R. J.; Wilson, R. W.; Wood, C. M.

    Published in: In A. L. Val, V. M. F. Almeida-Val, & D. J. Randall (Eds.), The Physiology of Tropical Fishes (Fish Physiology Vol. 21, pp. 397–442). Academic Press · 2005

    DOI: 10.1016/S1546-5098(05)21009-9

    • Book or book chapter
    • Verified

    Reviewed relationships: 1 partial support.

  6. Patterns of ion regulation in acidophilic fish native to the ion-poor, acidic Rio Negro

    Author or organization: Gonzalez, R. J.; Wilson, R. W.

    Published in: Journal of Fish Biology, 58(6), 1680–1690 · 2001

    DOI: 10.1111/j.1095-8649.2001.tb02322.x

    • Peer-reviewed journal article
    • Verified · citation corrected

    Reviewed relationships: 2 partial support.

  7. Environmental calcium regulates gill remodeling in a euryhaline teleost fish

    Author or organization: Platek, Alexis; Turko, Andy J.; Donini, Andrew; Kelly, Scott; Wright, Patricia A.

    Published in: Journal of Experimental Zoology Part A: Ecological and Integrative Physiology, 327(2–3), 139–142 · 2017

    DOI: 10.1002/jez.2079

    • Peer-reviewed journal article
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

  8. The effect of calcium on gill potentials and on sodium and chloride fluxes in the goldfish, Carassius auratus

    Author or organization: Eddy, Forrest Brian

    Published in: Journal of Comparative Physiology B, 96(2), 131–142 · 1975

    DOI: 10.1007/BF00706593

    • Peer-reviewed journal article
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

    Original package citation corrected during verification: the package named the journal as Journal of Comparative Physiology A; the paper was published in Journal of Comparative Physiology B.

  9. Effect of water pH and calcium on ion balance in five fish species of the Mekong Delta

    Author or organization: Yanagitsuru, Yuzo R.; Hewitt, Charles R.; Gam, Le Thi Hong; Phuong-Linh, Pham; Rodgers, Essie M.; Wang, Tobias; Bayley, Mark; Huong, Do Thi Thanh; Phuong, Nguyen Thanh; Brauner, Colin J.

    Published in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 232, 34–39 · 2019

    DOI: 10.1016/j.cbpa.2019.02.026

    • Peer-reviewed journal article
    • Verified · citation corrected

    Reviewed relationships: 1 partial support.

    Original package citation corrected during verification: the package attributed this paper to “Nguyen et al.”; the verified first author is Yuzo R. Yanagitsuru (Nguyen Thanh Phuong is a co-author).

  10. Effect of water calcium, copper, and silver on branchial Na⁺ permeability in a characid and cichlid fish

    Author or organization: Ambach, A.; Celo, K.; Lim, M.; Marbach, J.; Tikekar, Z.; Gonzalez, R. J.

    Published in: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 288, 111553 · 2024

    DOI: 10.1016/j.cbpa.2023.111553

    • Peer-reviewed journal article
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

    Original package citation corrected during verification: the package attributed this paper to “Duarte et al.” with no year; the verified paper is Ambach et al., published in 2024.

  11. Channels, pumps, and exchangers in the gill and kidney of freshwater fishes: their role in ionic and acid-base regulation

    Author or organization: Perry, S. F.; Shahsavarani, A.; Georgalis, T.; Bayaa, M.; Furimsky, M.; Thomas, S. L. Y.

    Published in: Journal of Experimental Zoology Part A: Comparative Experimental Biology, 300A(1), 53–62 · 2003

    DOI: 10.1002/jez.a.10309

    • Peer-reviewed journal article
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

  12. Calcium regulation of tight junction permeability

    Author or organization: Bleich, Markus; Shan, Qixian; Himmerkus, Nina

    Published in: Annals of the New York Academy of Sciences, 1258(1), 93–99 · 2012

    DOI: 10.1111/j.1749-6632.2012.06539.x

    • Peer-reviewed journal article
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

  13. The two faces of DOC

    Author or organization: Wood, Chris M.; Al-Reasi, H. A.; Smith, D. Scott

    Published in: Aquatic Toxicology, 105(3–4 Supplement), 3–8 · 2011

    DOI: 10.1016/j.aquatox.2011.03.007

    • Peer-reviewed journal article
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

  14. Mechanisms of ion transport in Potamotrygon, a stenohaline freshwater elasmobranch native to the ion-poor blackwaters of the Rio Negro

    Author or organization: Wood, Chris M.; Matsuo, Aline Y. O.; Gonzalez, R. J.; Wilson, Rod W.; Patrick, Marjorie L.; Val, Adalberto Luis

    Published in: Journal of Experimental Biology, 205(19), 3039–3054 · 2002

    DOI: 10.1242/jeb.205.19.3039

    • Peer-reviewed journal article
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

Veterinary & professional guidance

6 sources

Clinical references and veterinary conference proceedings written for veterinarians and fish-health professionals. Useful authoritative guidance, but not controlled experiments.

Show all 6 sources in Veterinary & professional guidance
  1. Environmental Diseases of Aquatic Animals in Aquatic Systems

    Author or organization: Francis-Floyd, Ruth; Yanong, Roy P. E.; Petty, Barbara D.

    Published in: Merck Veterinary Manual — Exotic and Laboratory Animals — Aquatic Systems · 2026

    • Veterinary reference
    • Verified · citation corrected

    Reviewed relationships: 6 direct support; 1 partial support.

  2. Disorders and Diseases of Fish

    Author or organization: Francis-Floyd, Ruth; Petty, Barbara D.

    Published in: Merck Veterinary Manual (Pet Owner Version) — All Other Pets — Fish · 2026

    • Veterinary reference
    • Verified · citation corrected

    Reviewed relationships: 4 direct support.

  3. Management of Aquarium Fish

    Author or organization: Petty, Barbara D.; Francis-Floyd, Ruth; Yanong, Roy P. E.

    Published in: Merck Veterinary Manual (Professional Version) — Exotic and Laboratory Animals — Aquarium Fish · 2026

    • Veterinary reference
    • Verified · citation corrected

    Reviewed relationships: 2 direct support.

  4. Equipment Needed for Aquatic Systems and Water Analysis (with tables 'A Summary of Water Quality Tests and Recommended Use' and '“Normal” Reference Ranges for Routine Water Quality Analysis')

    Author or organization: Francis-Floyd, Ruth; Yanong, Roy P. E.; Petty, Barbara D.

    Published in: Merck Veterinary Manual (Professional Version) — Exotic and Laboratory Animals — Aquatic Systems · 2026

    • Veterinary reference
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

  5. Water Quality Assessment in Aquatic Vet Medicine

    Published in: Proceedings of the 43rd World Small Animal Veterinary Association Congress and 9th FASAVA Congress (World Small Animal Veterinary Association; hosted on VIN) · 2018

    • Veterinary conference proceedings
    • Verified · citation corrected

    Reviewed relationships: 2 direct support.

  6. Introduction to pet fish medicine and surgery (Proceedings)

    Author or organization: Lewbart, Gregory A.

    Published in: dvm360 · 2011

    • Veterinary conference proceedings
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

Aquaculture, extension & fisheries references

7 sources

University extension fact sheets, aquaculture books and trade-magazine articles developed for ponds and recirculating fish farms. The chemistry transfers to aquariums; the exact numbers often do not.

Show all 7 sources in Aquaculture, extension & fisheries references
  1. Interactions of pH, Carbon Dioxide, Alkalinity and Hardness in Fish Ponds

    Author or organization: Wurts, William A.; Durborow, Robert M.

    Published in: Southern Regional Aquaculture Center Publication No. 464 · 1992

    • University extension publication
    • Verified

    Reviewed relationships: 1 direct support; 3 partial support.

  2. Liming Ponds for Aquaculture

    Author or organization: Wurts, William A.; Masser, Michael P.

    Published in: Southern Regional Aquaculture Center Publication No. 4100 (Revision, August 2013) · 2013

    • University extension publication
    • Verified · citation corrected

    Reviewed relationships: 1 direct support; 1 partial support.

    Original package citation corrected during verification: the package’s historical URL used the filename “Lining Ponds for Aquaculture”, while the publication’s title is “Liming Ponds for Aquaculture”. The link below is the verified copy of the August 2013 revision.

  3. Alkalinity and Hardness in Production Ponds

    Author or organization: Wurts, William A.

    Published in: World Aquaculture, 33(1), 16–17 · 2002

    • Professional / trade association
    • Verified · citation corrected

    Reviewed relationships: 1 citation mismatch / unsupported.

    This is a short trade-magazine article (World Aquaculture Society), not a peer-reviewed study. It is a real, verified source, but it does not contain the reproductive claim the original package attributed to it — see Important limitations. No verified public URL is recorded.

  4. Water Quality in Warmwater Fish Ponds

    Author or organization: Boyd, Claude E.

    Published in: Auburn University Agricultural Experiment Station · 1979

    • Book or book chapter
    • Verified

    Listed in the package bibliography only — not tied to a specific statement in the package.

  5. Carbon dioxide

    Author or organization: Tucker, C. S.

    Published in: In T. L. Wellborn, Jr. & J. R. MacMillan (Eds.), For Fish Farmers 84-2. Mississippi Cooperative Extension Service · 1984

    • University extension publication
    • Could not be independently verified

    Listed in the package bibliography only — not tied to a specific statement in the package.

    Could not be independently verified. This publication is cited by the verified SRAC Publication No. 464, which supports that it exists, but the original 1984 document could not be located and inspected.

  6. Recirculating Aquaculture

    Author or organization: Timmons, Michael B.; Guerdat, Todd; Vinci, Brian J.

    Published in: Ithaca Publishing Company LLC (NRAC Publication No. 401-2018) · 2018

    • Book or book chapter
    • Verified · limited scope

    Reviewed relationships: 1 historical attribution still limited.

    Verified with a scope limitation. The book’s identity (authors, 4th edition, publisher, year) was confirmed, but the exact 4th-edition passage used for the nitrification figures was not directly inspected — see Important limitations.

  7. Liming Farm and Recreational Ponds

    Author or organization: Crosby, David; Nerrie, Brian; Gregg, Cynthia L.

    Published in: Virginia Cooperative Extension Publication CNRE-126NP (Virginia State University; Virginia Cooperative Extension) · 2026

    • University extension publication
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

Government & water-treatment references

6 sources

Geological-survey pages on water hardness and wastewater-treatment guidance on nitrification. Reliable for chemistry and definitions; not written about fish.

Show all 6 sources in Government & water-treatment references
  1. Hardness of Water

    Author or organization: Water Science School, U.S. Geological Survey

    Published in: U.S. Geological Survey — Water Science School · 2026

    • Government agency
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

  2. Do you have information about water hardness in the United States?

    Author or organization: U.S. Geological Survey

    Published in: U.S. Geological Survey — FAQ (verified replacement location: official USGS legacy FAQ page, water.usgs.gov/owq/FAQ.htm) · 2025

    • Government agency
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

    The URL given in the original package no longer works. The same question and answer were verified on this official USGS page instead.

  3. Large subsurface wastewater treatment system process control monitoring for nitrogen

    Author or organization: Minnesota Pollution Control Agency

    Published in: Minnesota Pollution Control Agency · 2018

    • Government agency
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

    Original package citation corrected during verification: the package used a shortened title; the document’s official title is shown here.

  4. Long Island Sound Nitrogen Removal Training Program — Module 4: Fixed Film Operational Strategies

    Author or organization: New York State Department of Environmental Conservation

    Published in: New York State Department of Environmental Conservation · 1997

    • Government agency
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

  5. How Alkalinity Affects Nitrification

    Author or organization: Evans, Mary; Sober, Gary

    Published in: California Water Environment Association (current host); originally Water Environment Federation / WEF Headquarters, June 2015 · 2015

    • Professional / trade association
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

  6. Hardness

    Author or organization: Maryland Geological Survey

    Published in: Maryland Geological Survey

    • Government agency
    • Verified

    Listed in the package bibliography only — not tied to a specific statement in the package.

Aquarium organizations & publications

4 sources

Trade-association guidance and established hobby publications, cited for practical aquarium context. Helpful and informed, but not independent scientific evidence.

Show all 4 sources in Aquarium organizations & publications
  1. How to test water quality in your freshwater tank / aquarium

    Author or organization: Ornamental Aquatic Trade Association Ltd (OATA)

    Published in: Ornamental Aquatic Trade Association Ltd (OATA)

    • Professional / trade association
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

  2. CO2: Striking the balance

    Author or organization: Farmer, George

    Published in: Practical Fishkeeping · 2016

    • Aquarium hobby publication
    • Verified · citation corrected

    Reviewed relationships: 1 direct support.

  3. The wrong way to read the pH/KH chart.

    Author or organization: The 2Hr Aquarist

    Published in: The 2Hr Aquarist

    • Aquarium hobby publication
    • Verified

    Reviewed relationships: 1 direct support.

  4. 15.6.2. KH pH CO2 Relationships; 14.2.3. Buffering Substrates

    Author or organization: Aquarium Science

    Published in: Aquarium Science (aquariumscience.org)

    • Aquarium hobby publication
    • Verified · citation corrected

    Listed in the package bibliography only — not tied to a specific statement in the package.

Manufacturer documentation

2 sources

Product pages from the companies that make these products. They verify what a manufacturer says about its own product — they are not independent testing or scientific validation.

Show all 2 sources in Manufacturer documentation
  1. Equilibrium (product documentation)

    Author or organization: Seachem Laboratories, Inc.

    Published in: Seachem Laboratories, Inc. (manufacturer)

    • Manufacturer documentation
    • Verified · citation corrected

    Reviewed relationships: 3 manufacturer documentation.

    Verified as the manufacturer’s own product documentation. Dosing figures are the manufacturer’s claims, not independent test results.

  2. Bee Shrimp Mineral GH+ (product documentation)

    Author or organization: SaltyShrimp

    Published in: SaltyShrimp (manufacturer)

    • Manufacturer documentation
    • Verified · citation corrected

    Reviewed relationships: 2 manufacturer documentation.

    Verified as the manufacturer’s own product documentation. Dosing figures are the manufacturer’s claims, not independent test results.

Hobby & community context

4 sources

Forum discussions and specialist hobby resources. The original package itself states these were used to identify recurring questions and practices, not as scientific evidence.

Show all 4 sources in Hobby & community context
  1. Accuracy of pH/KH relation for CO₂ (discussion thread)

    Published in: Aquatic Plant Central (forum)

    • Hobbyist forum discussion
    • Could not be independently verified

    Reviewed relationships: 1 historical attribution still limited.

    Could not be independently verified. The original forum thread could not be recovered. The package used it as hobby context, not as scientific evidence.

  2. CO2/pH/KH table

    Author or organization: Tom Barr (thread starter) and forum participants

    Published in: Barr Report Forum — Aquarium Plants · 2013

    • Hobbyist forum discussion
    • Verified · citation corrected

    Reviewed relationships: 1 background support.

  3. pH relationship and the KH on a chart

    Published in: Aquarium Forum

    • Hobbyist forum discussion
    • Could not be independently verified

    Reviewed relationships: 2 historical attribution still limited.

    Could not be independently verified. The original forum thread could not be recovered, so the peat/atomic-absorption observation linked to it remains unresolved — see Important limitations.

  4. Specialist shrimp-keeping resources (unnamed group)

    • Unnamed group of sources
    • Could not be independently verified

    Reviewed relationships: 3 historical attribution still limited.

    Could not be independently verified. The package referred to “specialist shrimp-keeping resources” as a group without naming the individual sites, so they cannot be identified or checked.

Important limitations

The audit is complete, but it did not resolve everything. These are the gaps that remain.

  1. Four bibliography sources could not be independently verified

    • Carbon dioxide (Tucker, C. S., 1984)
    • Accuracy of pH/KH relation for CO₂ (discussion thread)
    • pH relationship and the KH on a chart
    • Specialist shrimp-keeping resources (unnamed group)

    Tucker (1984) is cited in the verified SRAC Publication No. 464, which supports that it exists, but the original 1984 publication was not located. The two forum threads could not be recovered, and the shrimp-keeping resources were never named individually in the package.

  2. Timmons et al. (2018), Recirculating Aquaculture, was verified with a scope limitation

    The book’s bibliographic identity was verified, but the exact 4th-edition passage used for the nitrification stoichiometry (how much alkalinity nitrification consumes) was not directly inspected. The same figures are corroborated by the wastewater and aquaculture sources in this record, but this particular book-to-claim link is not treated as fully verified.

  3. Wurts (2002) does not support one claim attributed to it

    The package cited Alkalinity and Hardness in Production Ponds for a claim that swings in alkalinity may disturb hormonal regulation, gonad development and gamete production. Independent verification found that this article does not contain that reproductive mechanism. It discusses alkalinity, hardness, pH swings, stress, growth and pond productivity instead.

    The historical citation is preserved, but this specific claim should not be treated as externally supported by Wurts 2002. No replacement source has been substituted.

  4. The peat / atomic-absorption claim remains unresolved

    The package described an observation that peat-filtered water tests softer on a hardness titration while calcium measured by atomic absorption stays unchanged. That observation came from a hobby forum thread that could not be recovered, and it has not been traced to a peer-reviewed source. It should not be read as established evidence.

  5. Shrimp parameter ranges cannot be independently audited

    The package compared commonly cited shrimp GH, KH, pH and TDS ranges using an unnamed group of specialist shrimp-keeping resources. The individual sources did not survive in the package, so that numerical comparison cannot be checked. The package itself labelled these targets as hobby convention.

  6. Manufacturer documentation is not independent testing

    The Seachem Equilibrium and SaltyShrimp Bee Shrimp Mineral GH+ pages were verified as the manufacturers’ own documentation. That confirms what each company says about its product, including dosing figures — not that those claims were independently tested.

More broadly: not every source is peer-reviewed aquarium research. Much of the physiology research studies wild or model species, and aquaculture and wastewater figures come from ponds and treatment plants rather than glass tanks.

What we did not do

  • We did not silently rewrite historical citations. The original package wording is preserved alongside every correction.
  • We did not replace missing sources with convenient modern substitutes.
  • We corrected source details (authors, journals, years, URLs) in our research registry while keeping a record of what the original package said.
  • We did not upgrade a source’s standing just because it was on the right topic.
  • We did not hide unavailable references — they remain listed and marked as unavailable.

How this record was built

  1. The Tank Guide assembled a master GH/KH/pH research package for content development.
  2. The package’s bibliography was extracted into The Tank Guide’s research registry — 43 sources and 68 source-to-claim relationships.
  3. Every bibliography source received an independent verification result.
  4. Source-to-claim relationships were reviewed separately from the package’s own evidence labels.
  5. Corrections and unresolved citations were preserved rather than hidden.
  6. The audit was completed on .

The Tank Guide did not conduct the external scientific studies listed here. FishKeepingLifeCo, publisher of The Tank Guide, compiled, reviewed and organized the research; the studies and references belong to their respective authors and publishers.